Study type: In vivo/preclinical · Status: Verified against declared source

Aging, mitochondrial dysfunction, and cerebral microhemorrhages: a preclinical evaluation of SS-31 (elamipretide) and development of a high-throughput machine learning-driven imaging pipeline for cerebromicrovascular protection therapeutic screening.

GeroScience · 2025

Study scale: The number of animals per group was determined based on our prior studies assessing CMH burden in aged hypertensive mice, where a similar sample size per group provided sufficient statistical power to detect significant differences using histological quantification [17, 18, 23, 35, 36, 63].

Abstract only: Open source record

Product or molecular entity relationships

  • SS-31: Exact entity relationship. Legacy citation custody associates this source with the catalog record; no product-relevance conclusion is implied.

Plain-language verified summary

Question

SS-31 (elamipretide) is a mitochondria-targeting tetrapeptide (d-Arg-2′,6′-dimethylTyr-Lys-Phe-NH2) that binds to cardiolipin, stabilizing mitochondrial membrane integrity, improving electron transport chain efficiency, and reducing mitochondrial ROS production [50–57].

Methods

Cerebral microhemorrhages (CMHs, also known as cerebral microbleeds) contribute to vascular cognitive impairment and dementia (VCID), with aging and hypertension being key risk factors.

Scale or participants

The number of animals per group was determined based on our prior studies assessing CMH burden in aged hypertensive mice, where a similar sample size per group provided sufficient statistical power to detect significant differences using histological quantification [17, 18, 23, 35, 36, 63].

Key findings

Cerebral microhemorrhages (CMHs, also known as cerebral microbleeds) contribute to vascular cognitive impairment and dementia (VCID), with aging and hypertension being key risk factors.

Limitations and uncertainty

While segmentation is highly efficient, taking only ~ 0.5 min per image (Table 1), whole-brain reconstruction, alignment, and rendering remain computationally intensive.

Verified against declared source. Verification is limited to the declared source and review scope. It does not mean independent replication or establish efficacy, safety, or suitability.