Study type: In vivo/preclinical · Status: Verified against declared source

Therapeutic Benefit of Extended Thymosin β4 Treatment Is Independent of Blood Glucose Level in Mice with Diabetic Peripheral Neuropathy.

Journal of diabetes research · 2015

Study scale: Intraepidermal nerve fiber profiles were digitized under a 40x objective (Carl Zeiss Axiostar Plus Microscope, USA) via the MCID system.

Abstract only: Open source record

Product or molecular entity relationships

Thymosin Beta-4 (TB500): Exact entity relationship. Legacy citation custody associates this source with the catalog record; no product-relevance conclusion is implied.

Plain-language verified summary

Question

For morphometric analysis of sciatic nerves, three sections spaced as 60 μm interval for each staining were used for analysis from each mouse, and three fields of the view per section were randomly imaged under a 100x oil immersion objective (BX40; Olympus Optical, Japan).

Methods

To examine the sensitivity to noxious heat, plantar test was measured using a thermal stimulation meter (IITC model 336 TG combination tail-flick and paw analgesia meter; IITC Life Science, USA) according to published methods [16].

Scale or participants

Intraepidermal nerve fiber profiles were digitized under a 40x objective (Carl Zeiss Axiostar Plus Microscope, USA) via the MCID system.

Key findings

Peripheral neuropathy is a chronic complication of diabetes mellitus.

Limitations and uncertainty

Peripheral neuropathy is a chronic complication of diabetes mellitus.

Verified against declared source. Verification is limited to the declared source and review scope. It does not mean independent replication or establish efficacy, safety, or suitability.