Study type: In vitro · Status: Verified against declared source

Quantitative real-time imaging of glutathione

2017

Study scale: Depending on the settings of cell imaging (including magnification, resolution, imaging length, imaging speed, laser power and stimulation method), the number of cells available for analysis varied from experiments.

Abstract only: Open source record

Product or molecular entity relationships

Glutathione: Exact entity relationship. Legacy citation custody associates this source with the catalog record; no product-relevance conclusion is implied.

Plain-language verified summary

Question

Michael addition reaction has been widely applied in organic synthesis and bioconjugation to build carbon–carbon and carbon–heteroatom bonds based on its forward reaction.

Methods

Glutathione plays many important roles in biological processes; however, the dynamic changes of glutathione concentrations in living cells remain largely unknown.

Scale or participants

Depending on the settings of cell imaging (including magnification, resolution, imaging length, imaging speed, laser power and stimulation method), the number of cells available for analysis varied from experiments.

Key findings

Fluorescent sensors for small biomolecules are needed to shed insight into real-time cellular processes.

Limitations and uncertainty

Glutathione plays many important roles in biological processes; however, the dynamic changes of glutathione concentrations in living cells remain largely unknown.

Verified against declared source. Verification is limited to the declared source and review scope. It does not mean independent replication or establish efficacy, safety, or suitability.