Study type: In vivo/preclinical · Status: Verified against declared source
The Role of T Cells Reactive to the Cathelicidin Antimicrobial Chemical sequence LL-37 in Acute Coronary Syndrome and Plaque Calcification.
Frontiers in immunology · 2020
Study scale: In samples with sufficient cell numbers, stimulated PBMCs cultured for 72 h were collected for RNA extraction using Trizol (Thermo Fisher) and subjected to qRT-PCR with SYBR green and a primer pair for human PDCD1.
Abstract only: Open source record
Product or molecular entity relationships
- LL-37: Exact entity relationship. Legacy citation custody associates this source with the catalog record; no product-relevance conclusion is implied.
Plain-language verified summary
Question
The human cationic anti-microbial peptide LL-37 is a T cell self-antigen in patients with psoriasis, who have increased risk of cardiovascular events.
Methods
In samples with sufficient cell numbers, stimulated PBMCs cultured for 72 h were collected for RNA extraction using Trizol (Thermo Fisher) and subjected to qRT-PCR with SYBR green and a primer pair for human PDCD1.
Scale or participants
In samples with sufficient cell numbers, stimulated PBMCs cultured for 72 h were collected for RNA extraction using Trizol (Thermo Fisher) and subjected to qRT-PCR with SYBR green and a primer pair for human PDCD1.
Key findings
The human cationic anti-microbial peptide LL-37 is a T cell self-antigen in patients with psoriasis, who have increased risk of cardiovascular events.
Limitations and uncertainty
The human cationic anti-microbial peptide LL-37 is a T cell self-antigen in patients with psoriasis, who have increased risk of cardiovascular events.
Verified against declared source. Verification is limited to the declared source and review scope. It does not mean independent replication or establish efficacy, safety, or suitability.