Study type: In vivo/preclinical · Status: Verified against declared source

HIF-1 mediated activation of antimicrobial chemical sequence LL-37 in type 2 diabetic patients.

Journal of molecular medicine (Berlin, Germany) · 2022

Study scale: TERT-NHUC and 5637 cells were pretreated with glucose (30 mM) with and without DMOG (400 µM) and incubated in hypoxia (1% O2) for at least 24 h followed by infection with 106 E.

Abstract only: Open source record

Product or molecular entity relationships

LL-37: Exact entity relationship. Legacy citation custody associates this source with the catalog record; no product-relevance conclusion is implied.

Plain-language verified summary

Question

Bladder sections were deparaffinized and rehydrated, pretreated with 0.3% Triton X-100 in PBS, or boiled in citrate buffer, 1 mM EDTA, 10 mM tris, 0.05% Tween 20 (pH 9).

Methods

The online version contains supplementary material available at 10.1007/s00109-021-02134-7.

Scale or participants

TERT-NHUC and 5637 cells were pretreated with glucose (30 mM) with and without DMOG (400 µM) and incubated in hypoxia (1% O2) for at least 24 h followed by infection with 106 E.

Key findings

Infections are common in patients with diabetes, but increasing antibiotic resistance hampers successful bacterial clearance and calls for alternative treatment strategies.

Limitations and uncertainty

Infections are common in patients with diabetes, but increasing antibiotic resistance hampers successful bacterial clearance and calls for alternative treatment strategies.

Verified against declared source. Verification is limited to the declared source and review scope. It does not mean independent replication or establish efficacy, safety, or suitability.