Study type: In vivo/preclinical · Status: Verified against declared source
Humanin activates integrin αV-TGFβ axis and leads to glioblastoma progression.
Cell death & disease · 2024
Study scale: a Representative images of filopodia formation upon 20 μM of humanin treatment for 24 h (left) and quantification for numbers of filopodia (right) in X02, 83, and 1123 cells.
Abstract only: Open source record
Product or molecular entity relationships
- Humanin: Exact entity relationship. Legacy citation custody associates this source with the catalog record; no product-relevance conclusion is implied.
Plain-language verified summary
Question
The protocol for this assay was adapted from a previous study [71].
Methods
Total RNA was extracted from cells using TRIzol reagent (Invitrogen, Cat# 15596018) following the manufacturer’s instructions.
Scale or participants
a Representative images of filopodia formation upon 20 μM of humanin treatment for 24 h (left) and quantification for numbers of filopodia (right) in X02, 83, and 1123 cells.
Key findings
The role of mitochondria peptides in the spreading of glioblastoma remains poorly understood.
Limitations and uncertainty
One of our intriguing observations is that, humanin appears to exhibit considerable stability, as evidenced by its detection in the brain by IHC staining several weeks after the initial injection.
Verified against declared source. Verification is limited to the declared source and review scope. It does not mean independent replication or establish efficacy, safety, or suitability.