Study type: In vivo/preclinical · Status: Verified against declared source

Thymosin beta-4 regulates activation of hepatic stellate cells via hedgehog signaling.

Scientific reports · 2017

Study scale: As GLI2 was down-regulated in the TB4-suppressed LX-2 cells, we assessed the subcellular distribution of GLI2 in these cells.

Abstract only: Open source record

Product or molecular entity relationships

Thymosin Beta-4 (TB500): Exact entity relationship. Legacy citation custody associates this source with the catalog record; no product-relevance conclusion is implied.

Plain-language verified summary

Question

Smotm2Amc/J (SMO-flox) mice were purchased from The Jackson Laboratory and male WT C57BL/6 mice were purchased from Hyochang (Dae-gu, Korea).

Methods

To generate TB4 knockout LX-2 cells or human pHSCs (purchased from Zen-Bio Inc., NC, USA), CRISPR/Cas9 mediated TB4 knockout was done according to the manufacturer’s instructions (Santa Cruz Biotechnology, Inc., CA, USA).

Scale or participants

As GLI2 was down-regulated in the TB4-suppressed LX-2 cells, we assessed the subcellular distribution of GLI2 in these cells.

Key findings

The molecular mechanisms of thymosin beta-4 (TB4) involved in regulating hepatic stellate cell (HSC) functions remain unclear.

Limitations and uncertainty

Thymosin beta-4 (TB4), an important G-actin sequestering protein, is the most abundant peptide of the beta-thymosin family20.

Verified against declared source. Verification is limited to the declared source and review scope. It does not mean independent replication or establish efficacy, safety, or suitability.