Study type: In vitro · Status: Verified against declared source

Entinostat up-regulates the CAMP gene encoding LL-37 via activation of STAT3 and HIF-1α transcription factors.

Scientific reports · 2016

Study scale: In order to determine which part of the regulatory region of the CAMP gene that might be involved in the Entinostat-mediated induction, in silico analysis of the CAMP promoter was performed.

Abstract only: Open source record

Product or molecular entity relationships

LL-37: Exact entity relationship. Legacy citation custody associates this source with the catalog record; no product-relevance conclusion is implied.

Plain-language verified summary

Question

Next, we asked whether increased expression of HIF-1α was reflected by a functional activation of this transcription factor.

Methods

For evaluation of induction on mRNA level, cells were seeded in 6-well plates and grown for 24 h before incubation with stimuli or vehicle for different time points.

Scale or participants

In order to determine which part of the regulatory region of the CAMP gene that might be involved in the Entinostat-mediated induction, in silico analysis of the CAMP promoter was performed.

Key findings

Bacterial resistance against classical antibiotics is a growing problem and the development of new antibiotics is limited.

Limitations and uncertainty

Bacterial resistance against classical antibiotics is a growing problem and the development of new antibiotics is limited.

Verified against declared source. Verification is limited to the declared source and review scope. It does not mean independent replication or establish efficacy, safety, or suitability.