Study type: In vitro · Status: Evidence verified by machine against declared source
Designing Analogs of SAAP-148 with Enhanced Antimicrobial and Anti-LPS Activities.
International journal of molecular sciences · 2024
Study scale: The geometric mean of minimum inhibitory concentration (MIC) values (GM) from the five strains was calculated to provide a comprehensive assessment of antimicrobial activity, as presented in Table 4.
Abstract only: Open source record
Product or molecular entity relationships
- LL-37: Exact entity relationship. Legacy citation custody associates this source with the catalog record; no product-relevance conclusion is implied.
Public plain-language summary
Some fields are not published, because the text held for them did not answer their heading. The published text is limited to the declared source and preserves reported uncertainty. It does not establish efficacy, safety, suitability, or evidence strength.
Research question
In this study, we employed Lys-scan to produce a series of amphiphilic SAAP-148 analogs derived from the SAAP-148 sequence to investigate the impact of the distribution of positively charged residues on the biological viability of the antimicrobial peptides (AMPs).
Study design
Not reported in the declared source.
Participants or experimental system
Nevertheless, their clinical application continues to pose challenges such as potential human toxicity, susceptibility to degradation under harsh environmental conditions (due to sensitivity to proteases and extreme pH), and lack of specificity for particular strains.
Study scale
The geometric mean of minimum inhibitory concentration (MIC) values (GM) from the five strains was calculated to provide a comprehensive assessment of antimicrobial activity, as presented in Table 4.
Intervention or exposure
Specifically, cell viability following treatment with 25 μM concentrations of SAAP-4, SAAP-7, and SAAP-148 was 96.88%, 98.22%, and 14.9%, respectively.
Comparator
The replacement of lysine led to a reduction in hemolytic activity compared to SAAP-148, with slightly higher haemolysis rates observed in SAAP-11 and SAAP-13.
Outcomes examined
The physical properties and biological activity of the designed peptides were subsequently compared.
Key findings
SAAP-148, a derivative of LL-37, exhibits a well-defined amphipathic structure and enhanced antimicrobial activity; however, it also displays significant cytotoxicity towards human cells.
Limitations and uncertainty
Not published. The text held for this field did not answer this heading, so nothing is shown here and nothing is substituted for it.
Product relevance and evidence boundary
This record is a published study, held here with its citation and review status. It is not a statement that any catalog item is effective, safe, or suitable for any use, and nothing in it is a dose or a protocol.
Evidence verified by machine against declared source. Verification is limited to the declared source and review scope. It does not mean independent replication or establish efficacy, safety, or suitability.