Study type: In vivo/preclinical · Status: Verified against declared source
Mitofusin 2 controls mitochondrial and synaptic dynamics of suprachiasmatic VIP neurons and related circadian rhythms.
The Journal of clinical investigation · 2025
Study scale: To determine the effect of the SCN circadian oscillation in the LD cycle at the cellular level, we first analyzed synapse density and mitochondrial morphology in SCN core VIP- expressing neurons of C57BL/6J mice at Zeitgeber times (ZT) 1, 7, 13, 19, (ZT0: lights on; ZT12: lights off).
Abstract only: Open source record
Product or molecular entity relationships
- VIP: Exact entity relationship. Legacy citation custody associates this source with the catalog record; no product-relevance conclusion is implied.
Plain-language verified summary
Question
All mice were maintained in temperature- and humidity-controlled rooms under a 12-hour light/12-hour dark cycle (lights on at 0700 hours, lights off at 1900 hours) unless otherwise stated.
Methods
To determine the effect of the SCN circadian oscillation in the LD cycle at the cellular level, we first analyzed synapse density and mitochondrial morphology in SCN core VIP- expressing neurons of C57BL/6J mice at Zeitgeber times (ZT) 1, 7, 13, 19, (ZT0: lights on; ZT12: lights off).
Scale or participants
To determine the effect of the SCN circadian oscillation in the LD cycle at the cellular level, we first analyzed synapse density and mitochondrial morphology in SCN core VIP- expressing neurons of C57BL/6J mice at Zeitgeber times (ZT) 1, 7, 13, 19, (ZT0: lights on; ZT12: lights off).
Key findings
Sustaining the strong rhythmic interactions between cellular adaptations and environmental cues has been posited as essential for preserving the physiological and behavioral alignment of an organism to the proper phase of the daily light/dark (LD) cycle.
Limitations and uncertainty
The daily fluctuation of SCN gene expression and neuronal firing requires precise rhythmic regulation of local metabolic activity to meet its bioenergetic demand.
Verified against declared source. Verification is limited to the declared source and review scope. It does not mean independent replication or establish efficacy, safety, or suitability.