Study type: In vitro · Status: Verified against declared source

GluN2C/D-containing NMDA receptors enhance temporal summation and increase sound-evoked and spontaneous firing in the inferior colliculus.

The Journal of physiology · 2025

Study scale: Slices were placed in a recording chamber and continuously perfused at a rate of ∼2 mL min–1 with 34°C aCSF bubbled in 5% CO2/95% O2.

Abstract only: Open source record

Product or molecular entity relationships

VIP: Exact entity relationship. Legacy citation custody associates this source with the catalog record; no product-relevance conclusion is implied.

Plain-language verified summary

Question

All experiments were approved by the University of Michigan Institutional Animal Care and Use Committee and were in accordance with NIH guidelines for the care and use of laboratory animals.

Methods

To apply glutamate puffs to brain slices, we pulled puffer pipettes from borosilicate glass (outer diameter 1.5 mm, inner diameter 0.86 mm; Sutter Instrument) to a resistance of 3.5–5.0 MΩ using a P‐1000 microelectrode puller and filled them with 300 µm or 1 mm glutamate (catalog.

Scale or participants

Slices were placed in a recording chamber and continuously perfused at a rate of ∼2 mL min–1 with 34°C aCSF bubbled in 5% CO2/95% O2.

Key findings

Along the ascending auditory pathway, there is a broad shift from temporal coding, which is common in the lower auditory brainstem, to rate coding, which predominates in auditory cortex.

Limitations and uncertainty

The inferior colliculus (IC) in the midbrain plays a critical role in auditory processing, functioning as a hub of integration in the auditory pathway (Adams, 1979; Oliver & Huerta, 1992) and as a site where phase‐locked ascending inputs are transformed into a firing rate code (Hewitt & Meddis, 1994; Joris et al., 2004).

Verified against declared source. Verification is limited to the declared source and review scope. It does not mean independent replication or establish efficacy, safety, or suitability.