Study type: In vivo/preclinical · Status: Verified against declared source

Systems spatiotemporal dynamics of traumatic brain injury at single-cell resolution reveals humanin as a therapeutic target.

Cellular and molecular life sciences : CMLS · 2022

Study scale: Mild traumatic brain injury (mTBI) or concussive injury comprises over 90% of the brain injuries in the United States and can lead to deficits in neuronal function and cognitive abilities that can persist for years after the initial incident [1–3].

Abstract only: Open source record

Product or molecular entity relationships

Humanin: Exact entity relationship. Legacy citation custody associates this source with the catalog record; no product-relevance conclusion is implied.

Plain-language verified summary

Question

We previously used single-cell RNA sequencing (scRNAseq) [13] to dissect the complex pathophysiology underlying mTBI in the heterogenous hippocampus tissue using a mild fluid percussion injury (FPI) mouse model [14].

Methods

The online version contains supplementary material available at 10.1007/s00018-022-04495-9.

Scale or participants

Mild traumatic brain injury (mTBI) or concussive injury comprises over 90% of the brain injuries in the United States and can lead to deficits in neuronal function and cognitive abilities that can persist for years after the initial incident [1–3].

Key findings

Our results offer a systems-level understanding of the dynamic and spatial regulation of gene programs by mTBI and pinpoint key target genes, pathways, and cell circuits that are amenable to therapeutics.

Limitations and uncertainty

We also identified pathways that showed both regional and dynamic specificity.

Verified against declared source. Verification is limited to the declared source and review scope. It does not mean independent replication or establish efficacy, safety, or suitability.