Study type: In vivo/preclinical · Status: Verified against declared source
Ablation of ferroptosis regulator glutathione peroxidase 4 in forebrain neurons promotes cognitive impairment and neurodegeneration
Redox Biology · 2017
Study scale: Mice were anesthetized then transcardially perfused with PBS followed by 4% paraformaldehyde.
Abstract only: Open source record
Product or molecular entity relationships
- Glutathione: Exact entity relationship. Legacy citation custody associates this source with the catalog record; no product-relevance conclusion is implied.
Plain-language verified summary
Question
Mice were anesthetized then transcardially perfused with PBS followed by 4% paraformaldehyde.
Methods
A PCR-based method was used to detect Cre-mediated recombination of the floxed Gpx4 allele in genomic DNA isolated from tissues.
Scale or participants
Mice were anesthetized then transcardially perfused with PBS followed by 4% paraformaldehyde.
Key findings
Synaptic loss and neuron death are the underlying cause of neurodegenerative diseases such as Alzheimer's disease (AD); however, the modalities of cell death in those diseases remain unclear.
Limitations and uncertainty
Synaptic loss and neuron death are the underlying cause of neurodegenerative diseases such as Alzheimer's disease (AD); however, the modalities of cell death in those diseases remain unclear.
Verified against declared source. Verification is limited to the declared source and review scope. It does not mean independent replication or establish efficacy, safety, or suitability.