Study type: In vivo/preclinical · Status: Verified against declared source

Bicarbonate Resensitization of Methicillin-Resistantto β-Lactam Antibiotics.

Antimicrobial agents and chemotherapy · 2019

Study scale: Recently, several groups have attempted to improve standardized AST by altering growth conditions to better reflect the host environment.

Abstract only: Open source record

Product or molecular entity relationships

LL-37: Exact entity relationship. Legacy citation custody associates this source with the catalog record; no product-relevance conclusion is implied.

Plain-language verified summary

Question

Not reported in the reviewed source.

Methods

MIC values obtained in standard CA-MHB for the five study strains were generally within 2-fold of those obtained in CA-MHB plus 100 mM Tris (CA-MHB-Tris; see Table S1 in the supplemental material).

Scale or participants

Recently, several groups have attempted to improve standardized AST by altering growth conditions to better reflect the host environment.

Key findings

Endovascular infections caused by methicillin-resistant Staphylococcus aureus (MRSA) are a major health care concern, especially infective endocarditis (IE).

Limitations and uncertainty

AST protocols for MRSA have been standardized by the CLSI and involve growth of bacterial samples in 2% NaCl cation-supplemented, nutrient-rich Mueller-Hinton broth (CA-MHB) (12, 13).

Verified against declared source. Verification is limited to the declared source and review scope. It does not mean independent replication or establish efficacy, safety, or suitability.