Study type: In vivo/preclinical · Status: Verified against declared source

Metformin Treatment Attenuates Brain Inflammation and Rescues PACAP/VIP Neuroactive chemical sequence Alterations in Mice Fed a High-Fat Diet.

International journal of molecular sciences · 2021

Study scale: Several studies have associated HFD with impairments in executive function and memory [7,8,9,10], as well as an increased incidence of mood disorders, such as anxiety [11,12] and depression [13,14].

Abstract only: Open source record

Product or molecular entity relationships

  • VIP: Exact entity relationship. Legacy citation custody associates this source with the catalog record; no product-relevance conclusion is implied.

Plain-language verified summary

Question

Mice were allowed 1 week to acclimate to housing conditions, during which time they were provided ad libitum access to standard laboratory chow and tap water.

Methods

RNA was extracted from the left prefrontal cortex, left half of the hypothalamus, left hippocampus, and left amygdala of the SC, HFD, and HFD + M mice group.

Scale or participants

Several studies have associated HFD with impairments in executive function and memory [7,8,9,10], as well as an increased incidence of mood disorders, such as anxiety [11,12] and depression [13,14].

Key findings

High-fat diet (HFD)-induced comorbid cognitive and behavioural impairments are thought to be the result of persistent low-grade neuroinflammation.

Limitations and uncertainty

To determine whether the anti-inflammatory cytokine IL-10 played a role in the pro- and anti-inflammatory activities of HFD and metformin treatment, respectively, we analyzed IL-10 mRNA expression by real-time qPCR.

Verified against declared source. Verification is limited to the declared source and review scope. It does not mean independent replication or establish efficacy, safety, or suitability.