Study type: In vitro · Status: Verified against declared source

Vasoactive Intestinal Chemical sequence modulates trophoblast-derived cell line function and interaction with phagocytic cells through autocrine pathways.

Scientific reports · 2016

Study scale: The vasoactive intestinal peptide (VIP) is a pleiotropic polypeptide with potent smooth muscle relaxing, vasodilating, pro-secretory and anti-inflammatory effects upon binding high affinity VPAC1 or VPAC2 receptors coupled to stimulatory G protein and adenylate cyclase activation and with lower affinity to PAC1 receptors78.

Abstract only: Open source record

Product or molecular entity relationships

  • VIP: Exact entity relationship. Legacy citation custody associates this source with the catalog record; no product-relevance conclusion is implied.

Plain-language verified summary

Question

To transfect Swan 71 cells with a VIP siRNA (Santa Cruz Biotechnology, Dallas, TX, USA), cells were grown at 60% of confluence and arrested for 3 h in Optimem®.

Methods

VIP receptors messenger RNA (mRNA) expression was analyzed by quantitative reverse transcription–polymerase chain reaction (qRT-PCR) as previously described2234.

Scale or participants

The vasoactive intestinal peptide (VIP) is a pleiotropic polypeptide with potent smooth muscle relaxing, vasodilating, pro-secretory and anti-inflammatory effects upon binding high affinity VPAC1 or VPAC2 receptors coupled to stimulatory G protein and adenylate cyclase activation and with lower affinity to PAC1 receptors78.

Key findings

Trophoblast cells migrate and invade the decidual stroma in a tightly regulated process to maintain immune homeostasis at the maternal-placental interface during the first weeks of pregnancy.

Limitations and uncertainty

To investigate whether both VPAC1 and VPAC2 receptor subtypes were involved indistinctively in the modulation of trophoblast cell function, we analyzed cell migration in HTR8 cells over-expressing either VPAC1 or VPAC2 receptors.

Verified against declared source. Verification is limited to the declared source and review scope. It does not mean independent replication or establish efficacy, safety, or suitability.