Study type: In vitro · Status: Verified against declared source
A Gq-Ca2+ axis controls circuit-level encoding of circadian time in the suprachiasmatic nucleus.
Neuron · 2013
Study scale: To monitor the activation of CREs across circadian time, SCN slices were transduced with LVs encoding a firefly-luciferase reporter controlled by a minimal synthetic promoter containing two CREs (LV:CRE-luc) and deprived of any other regulatory sequences.
Abstract only: Open source record
Product or molecular entity relationships
- VIP: Exact entity relationship. Legacy citation custody associates this source with the catalog record; no product-relevance conclusion is implied.
Plain-language verified summary
Question
Not reported in the reviewed source.
Methods
To address the relationship between SCN circuitry, cytosolic signals, CREs, and the TTFL, we used viral transduction to deliver to organotypic SCN slices bioluminescence- and fluorescence-based reporters of cytosolic circadian rhythms.
Scale or participants
To monitor the activation of CREs across circadian time, SCN slices were transduced with LVs encoding a firefly-luciferase reporter controlled by a minimal synthetic promoter containing two CREs (LV:CRE-luc) and deprived of any other regulatory sequences.
Key findings
To monitor the activation of CREs across circadian time, SCN slices were transduced with LVs encoding a firefly-luciferase reporter controlled by a minimal synthetic promoter containing two CREs (LV:CRE-luc) and deprived of any other regulatory sequences.
Limitations and uncertainty
In the absence of their specific ligand, Clozapine-N-Oxide (CNO), DREADD receptors are silent and accordingly, CRE activity exhibited unperturbed circadian cycles (Figure 2A; note compressed ordinate scale) and vehicle addition had no effect on CRE circadian activation.
Verified against declared source. Verification is limited to the declared source and review scope. It does not mean independent replication or establish efficacy, safety, or suitability.