Study type: In vitro · Status: Verified against declared source
Antimicrobial Chemical sequence LL-37 Facilitates Intracellular Uptake of RNA Aptamer Apt 21-2 Without Inducing an Inflammatory or Interferon Response.
Frontiers in immunology · 2019
Study scale: Primary keratinocytes and fibroblasts from healthy donors were purchased from PromoCell or isolated from healthy volunteers respectively and were cultured as described previously (26).
Abstract only: Open source record
Product or molecular entity relationships
- LL-37: Exact entity relationship. Legacy citation custody associates this source with the catalog record; no product-relevance conclusion is implied.
Plain-language verified summary
Question
LL-37-complexed RNA has been reported in the literature to be less susceptible to degradation.
Methods
A significant finding of the work conducted in this study is the observation that LL-37-complexed aptamer is efficiently internalized by both keratinocytes and fibroblasts.
Scale or participants
Primary keratinocytes and fibroblasts from healthy donors were purchased from PromoCell or isolated from healthy volunteers respectively and were cultured as described previously (26).
Key findings
RNA aptamers are synthetic single stranded RNA oligonucleotides that function analogously to antibodies.
Limitations and uncertainty
RNA aptamers are synthetic single stranded RNA oligonucleotides that function analogously to antibodies.
Verified against declared source. Verification is limited to the declared source and review scope. It does not mean independent replication or establish efficacy, safety, or suitability.