Study type: Human research · Status: Verified against declared source

Oral intake of phenylbutyrate with or without vitamin D3 upregulates the cathelicidin LL-37 in human macrophages: a dose finding study for treatment of tuberculosis.

BMC pulmonary medicine · 2013

Study scale: Healthy participants (age range 18 to 55 yrs; n = 15) working at International Centre for Diarrheal Disease Research, Bangladesh (icddr,b) and National Institute of the Diseases of the Chest and Hospital (NIDCH), Dhaka, Bangladesh were recruited in this study.

Abstract only: Open source record

Product or molecular entity relationships

  • LL-37: Exact entity relationship. Legacy citation custody associates this source with the catalog record; no product-relevance conclusion is implied.

Plain-language verified summary

Question

We earlier showed that 4-phenylbutyrate (PB) can induce cathelicidin LL-37 expression synergistically with 1,25-dihydroxyvitamin D3 in a lung epithelial cell line.

Methods

Healthy volunteers were enrolled in an 8-days open trial with three doses of PB [250 mg (Group-I), 500 mg (Group-II) or 1000 mg (Group-III)] twice daily (b.d.) together with vitamin D3 {5000 IU once daily (o.d.)}, PB (500 mg b.d.) (Group-IV) or vitamin D3 (5000 IU o.d.) (Group-V), given orally for 4 days.

Scale or participants

Healthy participants (age range 18 to 55 yrs; n = 15) working at International Centre for Diarrheal Disease Research, Bangladesh (icddr,b) and National Institute of the Diseases of the Chest and Hospital (NIDCH), Dhaka, Bangladesh were recruited in this study.

Key findings

MDM from Group-II had increased concentration of LL-37 peptide and transcript at day-4, while Group-I showed increased transcript at day-4 and day-8 compared to day-0 (p < 0.05).

Limitations and uncertainty

The average of 25-hydroxyvitamin D3 levels in plasma increased in all groups except for Group-IV (treated with only PB).

Verified against declared source. Verification is limited to the declared source and review scope. It does not mean independent replication or establish efficacy, safety, or suitability.