Study type: In vitro · Status: Verified against declared source
Agonists that stimulate secretion promote the recruitment of CFTR into membrane lipid microdomains.
The Journal of general physiology · 2019
Study scale: Subconfluent pHBE cells on glass coverslips were transduced with EGFP-CFTR and studied at 37°C in a humidified incubator in 5% CO2/95% air (Live Cell Instrument) on the stage of the confocal microscope as described previously (Abu-Arish et al., 2015).
Abstract only: Open source record
Product or molecular entity relationships
- VIP: Exact entity relationship. Legacy citation custody associates this source with the catalog record; no product-relevance conclusion is implied.
Plain-language verified summary
Question
Subconfluent pHBE cells on glass coverslips were transduced with EGFP-CFTR and studied at 37°C in a humidified incubator in 5% CO2/95% air (Live Cell Instrument) on the stage of the confocal microscope as described previously (Abu-Arish et al., 2015).
Methods
Details regarding the spatial ICS and k-space ICS (kICS) analyses are found in the supporting materials and references (Wiseman and Petersen, 1999; Kolin et al., 2006).
Scale or participants
Subconfluent pHBE cells on glass coverslips were transduced with EGFP-CFTR and studied at 37°C in a humidified incubator in 5% CO2/95% air (Live Cell Instrument) on the stage of the confocal microscope as described previously (Abu-Arish et al., 2015).
Key findings
The cystic fibrosis transmembrane conductance regulator (CFTR) is a tightly regulated anion channel that mediates secretion by epithelia and is mutated in the disease cystic fibrosis.
Limitations and uncertainty
The cystic fibrosis transmembrane conductance regulator (CFTR) is a tightly regulated anion channel that mediates secretion by epithelia and is mutated in the disease cystic fibrosis.
Verified against declared source. Verification is limited to the declared source and review scope. It does not mean independent replication or establish efficacy, safety, or suitability.